Publications
(*= corresponding author(s)
2023
Herwig, S., Kleinschmidt, J.H.*, 2023. The Formation of β-Strand Nine (β9) in the Folding and Insertion of BamA from an Unfolded Form into Lipid Bilayers. Membranes 13, 1-21. Abstract (Pubmed). Complete Paper (PDF)
2022
Pocanschi, C.L., Kleinschmidt, J. H.*, 2022. The Thermodynamic Stability of Membrane Proteins in Micelles and Lipid Bilayers Investigated with the Ferrichrom Receptor FhuA. J. Membr. Biol. 2022. Abstract (Pubmed). Complete Paper (PDF)
2019
Gerlach, L., Gholami, O., Schürmann, N., Kleinschmidt, J. H.*. 2019. Folding and insertion of β-barrel membrane proteins into lipid membranes by fluorescence spectroscopy. Meth. Mol. Biol. 2003, 465-492.
Schüßler, A., Herwig, S., Kleinschmidt, J. H.*. 2019. Kinetics of Insertion and Folding of Outer Membrane Proteins by Gel-Electrophoresis. Meth. Mol. Biol. 2003, 145-162.
Lipid-Protein Interactions: Methods and Protocols, 2nd extended edition 2019, 682 pages. Editor: Kleinschmidt, J. H., Meth. Mol. Biol., Vol 2003, Springer Protocols, Humana Press, ISBN 978-1-4939-9511-0. (series editor: Walker, J. M.)
2015
Kleinschmidt, J. H.*, 2015, Folding of β-barrel membrane proteins in lipid bilayers — Unassisted and assisted folding and insertion. Biochim. Biophys. Acta Biomembranes1848. 1927-1943 (http://dx.doi.org/10.1016/j.bbamem.2015.05.004).
Abstract (Pubmed). Complete Paper (PDF)
2014
Kleinschmidt, J. H.* and Popot, J.-L., 2014, Folding and stability of integral membrane proteins in amphipols. Arch. Biochem. Biophy 564, 327-343.
Abstract (Pubmed). Complete Paper (PDF)
2013
Patel, G. J., and Kleinschmidt, J. H.*, 2013, The Lipid-Bilayer Inserted Membrane Protein BamA of Escherichia coli facilitates Insertion and Folding of Outer Membrane Protein A from its Complex with Skp. Biochemistry 52, 3974-3986
Abstract (Pubmed). Complete Paper (PDF)
Lipid-Protein Interactions: Methods and Protocols, 2013. Editor: Kleinschmidt, J. H., Meth. Mol. Biol., Vol 974, Humana Press/Springer Protocols, ISBN 978-1-62703-274-2.
Pocanschi, C. L. •, Popot, J.-L., and Kleinschmidt, J. H.*, 2013. Folding and stability of outer membrane protein A (OmpA) from Escherichia coli in an amphipathic polymer, amphipol A8-35. Eur. Biophys. J. 42, 103-118.
Abstract (Pubmed). Complete Paper (PDF)
2012
Walser, R., Kleinschmidt, J. H., Skerra, A., Zerbe, O.* 2012. β-Barrel scaffolds for the grafting of extracellular loops from GPCRs. Biol. Chem. 393, 1341-1355.
Abstract (Pubmed). Complete Paper (PDF)
2011
Walser, R., Kleinschmidt, J. H., Zerbe, O.* 2011. A Chimeric GPCR Model Mimicking the Ligand Binding Site of the Human Y1 Receptor Studied by NMR Spectroscopy. ChemBioChem12, 1690–1693.
Abstract (Pubmed). Complete Paper (PDF)
Popot, J.-L.*, Althoff, T., Bagnard, D., Baneres, J. L., Bazzacco, P., Billon-Denis, E., Catoire, L. J., Champeil, P., Charvolin, D., Cocco, M. J., Cremel, G., Dahmane, T., de la Maza, L. M., Ebel, C., Gabel, F., Giusti, F., Gohon, Y., Goormaghtigh, E., Guittet, E., Kleinschmidt, J. H., Kühlbrandt, W., Le Bon, C., Martinez, K. L., Picard, M., Pucci, B., Sachs, J. N., Tribet, C., van Heijenoort, C., Wien, F., Zito, F., and Zoonens, M. , 2011, Amphipols from a to z*, Ann. Rev. Biophys. 40, 379-408
Abstract (Pubmed). Complete Paper (PDF)
Kleinschmidt*, J.H. Bulieris, P.V., Qu, J., Dogterom, M., den Blaauwen, T. 2011, Association of neighboring β-strands of outer membrane protein A in lipid bilayers revealed by site directed fluorescence quenching J. Mol. Biol. 407, 316-332
Abstract (Pubmed). Complete Paper (PDF)
2009
Patel, G. J., Behrens-Kneip, S., Holst, O., and Kleinschmidt, J. H.*, 2009,The periplasmic chaperone Skp facilitates targeting, insertion and folding of OmpA into lipid membranes with a negative membrane surface potential. Biochemistry, 48, 10235-10245
Abstract (Pubmed). Complete Paper (PDF)
Qu, J., Behrens-Kneip, S., Holst, O., and Kleinschmidt, J. H.*, 2009, Binding Regions of Outer Membrane Protein A in Complexes with the Periplasmic Chaperone Skp. A Site-Directed Fluorescence Study. Biochemistry, 48, 4926-4936
Abstract (Pubmed). Complete Paper (PDF)
2008
V. Anbazhagan, Vijay, N., Kleinschmidt, J. H.*, and Marsh D.*, 2008, Protein-Lipid Interactions with Fusobacterium nucleatum major outer membrane protein FomA: spin-label EPR and polarized infrared spectroscopy. Biochemistry, 47, 8414-8423
Abstract (Pubmed). Complete Paper (PDF)
Nesper, J., Brosig, A., Ringler, P., Patel, G. J., Müller, S., Kleinschmidt, J. H., Boos, W., Diederichs, K., Welte, W., 2008, TtOmp85 from Thermus thermophilus HB27: An ancestral type of the Omp85 family. J. Bacteriol. 190, 4568-4575.
Abstract (Pubmed). Complete Paper (PDF)
V. Anbazhagan, Qu, J., Kleinschmidt, J. H.*, and Marsh D.*, 2008, Incorporation of outer membrane protein OmpG in lipid membranes. Protein-lipid interactions and β-barrel orientation. Biochemistry 47, 6189-6198
Abstract (Pubmed). Complete Paper (PDF)
2007
Qu, J., Mayer, C., Behrens, S., Holst, O., and Kleinschmidt, J. H.*, 2007, The trimeric periplasmic chaperone Skp of E. coli forms 1:1 complexes with outer membrane proteins via hydrophobic and electrostatic interactions. J. Mol. Biol. 374, 91-105.
Abstract (Pubmed). Complete Paper (PDF)
Shanmugavadivu, B., Apell, H.-J., Meins, T., Zeth, K., and Kleinschmidt, J. H.*, 2007, Correct folding of the β-barrel of the human membrane protein VDAC requires a lipid bilayer. J. Mol. Biol. 368, 66-78
Abstract (Pubmed). Complete Paper (PDF)
Kleinschmidt, J.H.*, 2007, Assembly of Integral Membrane Proteins from the Periplasm into the Outer Membrane. In: The Periplasm, Ed.: Ehrmann, M., ASM Press Washington D.C., p30-66, (ISBN-10: 1-55581-398-4). Complete Paper (PDF)
2006
Pocanschi, C. L., Dahmane, T., Gohon, Y., Rappaport, F., Apell, H.-J., Kleinschmidt, J. H.*, Popot, J.-L.*, 2006, Amphipathic Polymers: Tools to Fold Integral Membrane Proteins to their Active Form. Biochemistry 45, 13954-13961
Abstract (Pubmed). Complete Paper (PDF)
Pocanschi, C.L., Patel, G. J., Marsh, D.*, Kleinschmidt, J.H.*, Curvature elasticity and refolding of OmpA in large unilamellar vesicles. Biophys. J., 91, L75-L78
Abstract (Pubmed). Complete Paper (PDF)
Marsh, D., Shanmugavadivu, B., and Kleinschmidt, J.H., 2006. Membrane elastic fluctuations and the insertion and tilt of β-barrel proteins. Biophys. J. 91, 227-232
Abstract (Pubmed). Complete Paper (PDF)
Kleinschmidt, J.H.*, 2006. Folding kinetics of the outer membrane proteins OmpA and FomA into phospholipid bilayers. Chem. Phys. Lipids, 141, 30-47
Abstract (Pubmed). Complete Paper (PDF)
Pocanschi, C.L., Apell, H.-J., Puntervoll, P., Høgh, B., Jensen, H. B., Welte, W. and Kleinschmidt, J. H.*, 2006. The Major Outer Membrane Protein of Fusobacterium Nucleatum (FomA) Folds and Inserts into Lipid Bilayers via Parallel Folding Pathways. J. Mol. Biol., 355, 548-561
Abstract (Pubmed). Complete Paper (PDF)
2005
Mogensen , J.E., Kleinschmidt, J. H., Schmidt, M. A., Otzen, D.E., 2005, Misfolding of a Bacterial Autotransporter. Protein Sci. 14, 2814-2827
Abstract (Pubmed). Complete Paper (PDF)
Kleinschmidt, J.H.*, 2005, Folding and Stability of monomeric β-barrel Membrane proteins, In: Protein-Lipid Interactions: From Membrane Domains to Cellular Networks, Ed. Tamm, L.K. Wiley-VCH Weinheim, p27-56, (ISBN 3-527-31151-3)
PDF
Ramakrishnan M., Qu, J, Pocanschi, C. L., Kleinschmidt J. H.*, Marsh D*, 2005, Orientation of β-barrel protein OmpA and FhuA in lipid membranes. Chainlength dependence from infrared dichroism. Biochemistry 44, 3515-3523.
Abstract (Pubmed). Complete Paper (PDF)
2004
Ramakrishnan M., Pocanschi C. L., Kleinschmidt J.H.*, Marsh D.* 2004, Association of Spin-Labeled Lipids with β-Barrel Proteins from the Outer Membrane of Escherichia coli. Biochemistry 43, 11630-11636.
Abstract (Pubmed). Complete Paper (PDF).
2003
Kleinschmidt, J.H.*, 2003. Membrane protein folding on the example of outer membrane protein A (OmpA) of Escherischia coli. Cell. Mol. Life Sci., 60, 1547-1558.
Abstract (Pubmed). Complete Paper (PDF).
Kleinschmidt, J.H.*, 2003. Membrane Proteins, Cell. Mol. Life Sci. 60, 1527-1528.
Bulieris, P.V., Behrens, S., Holst, O., Kleinschmidt, J. H.*, 2003. Folding and insertion of the outer membrane protein OmpA is assisted by the chaperone Skp and by lipopolysaccharide. J. Biol. Chem. 278, 9092-9099. Abstract (Pubmed). Complete Paper (PDF).
2002
Kleinschmidt, J. H.*, Tamm, L.K., 2002b. Secondary and tertiary structure formation of the β-barrel membrane protein OmpA is synchronized and depends on membrane thickness. J. Mol. Biol. 324, 319-330. Abstract (Pubmed) Complete paper (PDF)
Marsh, D. Horvath, L.I., Swamy, M.J. Mantripragada, S., Kleinschmidt, J.H. 2002. Interactions of membrane-spanning proteins with peripheral and lipid-anchored membrane proteins. Mol. Membr. Biol. 19, 247-255. Abstract (Pubmed) Complete paper (PDF)
Kleinschmidt, J. H.*, Tamm, L. K., 2002a. Structural Transitions in Short-Chain Lipid Assemblies Studied by 31P-NMR Spectroscopy. Biophys. J. 83, 994-1003. Abstract (Pubmed) Complete paper (PDF)
Kleinschmidt, J.H.*, 2002. Folding and Membrane Insertion of a β-barrel membrane protein. Habilitation Thesis. University of Konstanz.
2001
Tamm, L.K., Arora, A., Kleinschmidt, J.H. 2001. Structure and Assembly of β-barrel Membrane Proteins. J. Biol. Chem. 276, 32399-32402. Abstract (Pubmed). Complete paper (PDF)
2000
Pali, T. L., Kleinschmidt, J. H., Powell, G. and Marsh, D. 2000. Non-Linear Electron Paramagnetic Resonance Studies of the Interaction of Cytochrome c Oxidase with Spin-Labeled Lipids in Gel-Phase Membranes. Biochemistry 39, 2355-2361. Abstract (Pubmed) Complete paper (PDF)
1999
Kleinschmidt, J. H., Wiener, M. C., Tamm L. K., 1999. Outer membrane protein A of Escherichia coli folds into detergent micelles, but not in the presence of monomeric detergent. Protein Sci. 10, 2065-2071. Abstract (Pubmed). Complete paper (PDF)
Kleinschmidt, J. H., Tamm, L. K., 1999. Time-Resolved Distance Determination by Tryptophan Fluorescence Quenching (TDFQ): Probing Intermediates during Membrane Protein Folding. Biochemistry 38, 4996-5005 Abstract (Pubmed). Complete paper (PDF).
Kleinschmidt, J. H., den Blaauwen, T., Driessen, A., Tamm, L. K., 1999. Outer Membrane Protein A of E. coli Inserts and Folds into Lipid Bilayers by a Concerted Mechanism. Biochemistry 38, 5006-5016. Abstract (Pubmed). Complete paper (PDF)
1998
Kleinschmidt, J. H., Powell, G. L., Marsh, D., 1998. Cytochrome c-induced Increase in Motionally Restricted Lipid in Reconstituted Cytochrome c Oxidase Membranes, Revealed by Spin-label ESR Spectroscopy. Biochemistry 37, 11579-11585. Abstract (Pubmed) Complete Paper (PDF)
1997
Kleinschmidt, J. H., Marsh, D., 1997. Interactions of Lysine Peptides with Phospholipid Bilayers. A Spin-Label ESR Study. Biophys. J. 73, 2546-2555. Abstract (Pubmed) Complete Paper (PDF)
Kleinschmidt, J. H., Mahaney, J. E., Thomas, D. D., Marsh D., 1997. Interactions of Bee Venom Melittin with Zwitterionic and Negatively Charged Phospholipid Bilayers. A Spin-Label ESR Study. Biophys. J.72, 767-778. Abstract (Pubmed) Complete Paper (PDF)
1996
Kleinschmidt, J. H., Tamm, L. K., 1996. Folding Intermediates of a β-Barrel Membrane Protein. Kinetic Evidence for a Multi-Step Membrane Insertion Mechanism. Biochemistry 35, 12993-13000. Abstract (Pubmed) Complete paper (PDF)
1994
Kleinschmidt, J. H.*, 1994. "Electron Spin Resonance Studies on Lipid-Peptide and Lipid-Protein Interactions in Biological and Model Membranes". Ph.D.-thesis. Publisher: Verlag Dr. C. Shaker (www.shaker.de), Aachen, 1994, ISBN: 3-861111-904-8, 162 pages, published in German language.
1992
Mahaney, J. E., Kleinschmidt, J. H., Marsh, D., Thomas, D. D., 1992. Effects of Melittin on Lipid-Protein Interactions in Sarcoplasmatic Reticulum Membranes. Biophys. J. 63, 1513-1522. Abstract (Pubmed) Complete Paper (PDF)
* = corresponding author
2006
Pocanschi, C. L., Apell, H.-J., Welte, W., Puntervoll, P., Høgh, B. Jensen, H., Kleinschmidt, J. H.*, 2006. The major outer membrane protein of Fusobacterium nucleatum (FomA) folds and inserts into lipid bilayers via parallel folding pathways. Biophysical Society Meeting, Salt Lake City, USA. Biophys. J. 90 (1,part 2), 491A.
Shanmugavadivu B. Apell, H.-J., Zeth, K, and Kleinschmidt, J.H.* 2006. Folding and Membrane Insertion of a Eukaryotic Porin, VDAC (human isoform 1) into Phospholipid Bilayers. Biophysical Society Meeting, Salt Lake City, USA. February 2006. Biophys. J. 90 (1,part 2), 491A.
2005
Kleinschmidt, J. H., 2005. Folding of the monomeric β-barrel membrane proteins OmpA from E.coli, FomA from F. nucleatum, and VDAC human isoform 1, Invited Talk at the Meeting “Protein-Lipid Interactions”, Dubrovnik, Croatia, 4.-6. October 2005
Kleinschmidt, J. H., 2005. Folding pathways of β-barrel membrane proteins. Invited Talk at the Meeting “The molecular interface of Membrane Proteins and Lipids”, Cosenza, Italy, 10.-12. September 2005
Kleinschmidt, J. H., 2005. Recent developments in exploring membrane protein insertion and folding of β-barrel membrane proteins. Invited Talk, University of Toulouse, France 23. June 2005
Kleinschmidt, J. H., 2005. Insertion and Folding of β-barrel Membrane Protein Folding of bacterial and mitochondrial β-barrel membrane proteins on the examples of OmpA, FomA, and VDAC. Invited Talk at the Meeting “Principles of Membrane Protein Folding and Stability” Lisbon, Portugal, 10. Mai 2005
Kleinschmidt, J. H., 2005. Insertion and Folding of β-barrel Membrane Proteins on the Example of OmpA. Invited Talk at the Max-Planck-Institute for Developmental Biology, Tübingen, Germany.
Pocanschi, C. L., Popot, J.-L., Apell, H.-J., Kleinschmidt, J. H.*, 2005. The outer membrane proteins OmpA and FomA can be refolded from a completely denatured state into an amphipathic polymer. Biophysical Society Meeting, Long Beach, USA. February 2005. Biophys. J. 88 (1, Part 2), 77A-77A.
Shanmugavadivu B. Apell, H.-J., Zeth, K, and Kleinschmidt, J.H.*, 2005 The voltage-gated anion selective channel VDAC spontaneously folds into lipid bilayers from a completely denatured state. Biophysical Society Meeting, Long Beach, USA. February 2005. Biophys. J. 88 (1, Part 2), 78A-78A.
2004
Kleinschmidt, J. H., 2004. Insertion and Folding of β-barrel Membrane Proteins on the Example of OmpA. Invited Talk am Biologischen Forschungszentrum, Szedged, Hungary.
Kleinschmidt, J. H., 2004. Folding and Stability of β-barrel Transmembrane Proteins. Talk at the University of Toulouse, France.
Kleinschmidt, J.H., 2004. Experimental Procedures to Study the Insertion and Folding of β-barrel Transmembrane Proteins. Invited Talk at the International Student school, Aalborg, Denmark.
Bulieris, P.V., Kleinschmidt, J.H., 2004. Folding and Insertion of the Outer Membrane Protein OmpA is Assisted by the Chaperone Skp and by Lipopolysaccharide. Biophysical Society Meeting, Baltimore, USA. Biophys. J. 86, 351A.
2003
Kleinschmidt, J.H., 2003. Membrane Protein Folding Studied by Fluorescence Spectroscopy. Invited Talk, Biozentrum Basel, Switzerland.
Pocanschi, C. L., Kleinschmidt, J.*, 2003. Detergent-Micelle Extraction Reduces the Thermodynamic Stability of the Integral Membrane Protein FhuA by more than 50%. Biophys. J. 84, 351A.
PosterPresentation, Biophysical Society Meeting, San Antonio, USA.
Kleinschmidt, J.H.* and Tamm, L.K., 2003. Secondary and Tertiary Structure Formation of the β-barrel Membrane Protein OmpA is Synchronized and Depends on Membrane Thickness. Biophys. J. 84, 350A.
Poster Presentation, Biophysical Society Meeting, San Antonio, USA.
2002
Kleinschmidt, J.H.*, 2002. Secondary and tertiary structure formation in β-barrel membrane proteins is synchronized and dependent on membrane thickness.
Talk at the International Workshop “Lipid/Peptide- Lipid/Protein Interactions”. / Frühjahrstagung der deutschen Gesellschaft für Biophysik, Sekt. Zellen, Membranen, Netzwerke, Gomadingen, Germany.
Pocanschi, C. L., Kleinschmidt, J. H.*, 2002. The ferric hydroxamate uptake protein component A (FhuA) of E. coli is stabilized by the N-terminal cork-domain and by the lipid bilayer. Biophys. J. 82, 527A.
Poster Presentation at the Biophyscial Society Meeting, San Francisco, USA and at the International workshop “Lipid/Peptide- Lipid/Protein Interactions”. / Frühjahrstagung der deutschen Gesellschaft für Biophysik, Sekt. Zellen, Membranen, Netzwerke, Gomadingen, Germany
Poster presentation at the Biophysical Society Meeting, San Francisco, USA 2002.
2001
Kleinschmidt, J.H.*: The Membrane Insertion and the Folding of Outer Membrane Protein A (OmpA) are synchronized.
Talk at the first COST Action D22 workshop “Protein-Lipid Supramolecular Assemblies”, Zagreb, Croatia 2001.
Pocanschi, C.L., Ködding, J., Kleinschmidt, J.H.*, Spectroscopic Studies on the Folding of the Ferric Hydroxamate Uptake Protein Component A (Fhua), 2001, Biophys. J. 80, 555a
Poster presentation at the Biophysical Society Meeting, Boston, 2001.
Kleinschmidt, J.H.* and Tamm, L.K.: Structure of Short-Chain Phospholipid Assemblies Investigated by 31P-NMR Spectroscopy, 2001, Biophys J. 80, 425a
Poster presentation at the Biophysical Society Meeting, Boston, 2001.
2000
Kleinschmidt, J.H.* and Tamm, L.K.: The Folding Kinetics of OmpA into Large Unilamellar Vesicles Are of Second Order And Depend on the Hydrophobic Thickness of the Lipid Bilayer. Biophys. J. 78, 2000, 160A
Poster presentation at the Biophysical Society Meeting, New Orleans, 2000.
1999
Kleinschmidt, J. H.*, Wiener, M. C., Tamm, L. K.: Refolding of Outer Membrane Protein A in Neutral or Zwitterionic Detergents Requires Micelles and does not Depend on the Detailed Chemical Structure of the Detergent. Biophys. J. 76, A106.
1998
Kleinschmidt, J. H.*, Tamm, L.K. 1998. Time-Resolved Trp-Fluorescence Quenching (TRFQ): Probing Intermediates during Membrane Protein Folding. Biophys. J. 74, A15.
Kleinschmidt, J. H.*, den Blaauwen, T., Driessen, A. J. M., Tamm, L.K. 1998. Time-Resolved Fluorescence Quenching of Single-Trp Mutants of Outer Membrane Protein A of E. coli. Biophys. J. 74, A306.
1997
Kleinschmidt, J. H.*, Tamm, L. K. 1997. Fluorescence Quenching studies on the Refolding and Membrane Insertion of Outer Membrane Protein A (Omp A) of E. Coli. Biophys. J. 72, A315.
Kleinschmidt, J. H.*, Powell, G. L. and Marsh, D. 1997. Interactions of Cytochrome c with reconstituted Cytochrome c Oxidase Membranes. Evidence for Increased Cytochrome c Oxidase Boundary Lipid Induced By Cytochrome c ? Biophys. J. 72, A401.
1996
Kleinschmidt, J. H.*, Tamm, L. K. 1996. Kinetic Studies on the Refolding and Membrane Insertion of Outer Membrane Protein A (Omp A) of E. Coli. Biophys. J. 70, A177.